yemenjmedYemen Journal of MedicinecoVolume 2 Issue 1MultidisciplinaryEnglishJanuary - April 2023 NNY20230510ArticleUtility of The MGIT 960 TB System For Recovery of MycobacteriaEnglishY3032Amara EstherUlasi1EnglishNNdubuisi ObioraNwachukwu2EnglishYReginald AzuOnyeagba3EnglishYSolomon NnannaUmeham4EnglishYAnuliAmadi5EnglishY10.32677/yjm.v2i1.3877Background and Objectives: Pulmonary tuberculosis remains a public health issue in Nigeria. The rapid diagnosis of pulmonary tuberculosis is essential for the early initiation of treatment and management of patients. The utility of the BACTEC MGIT 960 TB system was evaluated and compared with the Lowenstein Jensen (LJ) culture method for the recovery of Mycobacteria from sputum samples. Methods: A total of 2400 sputum samples submitted to the South East TB Zonal Reference Laboratory, Amachara Umuahia, Nigeria were tested. Samples were decontaminated using the standard N-Acetyl-L-Cysteine Sodium Hydroxide method and concentrated prior to processing. The processed samples were inoculated into both MGIT 960 tubes and LJ medium and incubated accordingly. Results: From all sputum samples, Mycobacteria were recovered from 201(8.4%) sputum samples by the MGIT 960 system and 175(7.3%) by LJ culture (P 0.014). The sensitivity for MGIT and LJ culture for mycobacteria were 95.0% and 80.1% respectively. Among the 201 MGIT-positive cultures, 127(63.2%) were identified as Mycobacterium tuberculosis complex (MBTC) and 74(36.8%) as Mycobacteria other than tuberculosis ( MOTT). The recovery rate of MTBC from LJ-positive samples was 84.0% and MOTT 16.0%. MGIT 960 identified more MOTT than LJ culture(P 0.045). The contamination rate associated with MGIT and LJ culture was 4.1% and 2.5% respectively(P 0.037). The time to detection of mycobacteria in MGIT 960 and LJ was 14.8 days and 33.2 days respectively. Conclusion:  MGIT 960 has good diagnostic accuracy. It provided a more rapid and higher recovery of all mycobacteria than the LJ culture.EnglishBACTEC MGIT 960, Comparison, Lowenstein Jensen, Mycobacterium tuberculosishttps://yemenjmed.com/admin/abstract?id=47References1619Gopalaswamy R, Sivakumar S, Rajesh M, et al. Of tuberculosis and non-tuberculous mycobacterial infections- a comparative analysis of epidemiology, diagnosis and treatment. J Biomed Sci 2020; 27: 1-7.Walis RS, Pai M, Menzies D, et al. Biomarkers and diagnosis for tuberculosis: progress, needs and translation into practice. Lancet 2010; 375: 1920- 1937.McNerney R, Daley P. Towards a point-of-care test for active tuberculosis: obstacles and opportunities. Nat Rev Microbiol 2011; 9: 204- 213.World Health Organization. Global Tuberculosis Control. 2011. Geneva, Switzerland.Munshi SK, Rahman F, Kamal SMM, et al. Comparison among different diagnostic methods used for detection of extra-pulmonary tuberculosis in Bangladesh. 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